Adoptive transfer of leukemia-specific TCR transgenic CD8+ T cells to H8-bcCML mice. Purified p14 CD8+ T cells (CD45.1+CD8+Vα2+) were adoptively transferred to H8-bcCML mice (●), to naive C57BL/6 control mice (♦), and to C57BL/6 mice infected with 104 pfu of LCMV (■). (A-B) The expansion of the transferred p14 CD8+ T cells (CD45.1+) was analyzed in the blood (A) and spleen (B) by flow cytometry analysis. (C) The expression of the leukemia-specific TCR Vα2 on the transferred p14 CD8+ T cells (CD45.1+) was analyzed in blood 3 days after transfer by flow cytometry analysis. (D) The expression of the TCR Vα2 was compared with the percentage of leukemic granulocytes (GFP+) in the blood 3 days after transfer. Results are pooled from 5 independent experiments. Statistical comparison was made using the Spearman correlation.
