Effector memory phenotype of tumor-specific human CD8⁺ T cells overexpressing IL-2, IL-7, IL-15, or IL-21. (A) CCR7 expression of CD8⁺ T cells 7 days after AAPC stimulation. Histograms have been gated on the CD8⁺CD45RA⁻CD45RO⁺ subset and are from 1 of 2 donors with similar results observed in both. Thick black line indicates 19z1⁺; thin black line, 19z1⁻; and gray line, isotype control. (B) CD62L expression of CD8⁺ T cells 7 days after AAPC stimulation. Histograms are representative of 8 donors and have been gated on the 19z1⁺CD8⁺CD45RA⁻CD45RO⁺ subset. The bar graph represents the average percentage (± SEM) of CD62L⁻ T cells within the 19z1⁺CD8⁺CD45RA⁻CD45RO⁺ subset from 8 donors. Histograms for individual experiments are shown in supplemental Figure 7. (C) CD27 and CD28 expression of CD8⁺ T cells 7 days after AAPC stimulation. Dot plots are representative of 4 donors and have been gated on the 19z1⁺CD8⁺ subset. The bar graph represents the average percentage (± SEM) of CD27⁺CD28⁺ (T_EM1), CD27⁺CD28⁻ (T_EM2), CD27⁻CD28⁻ (T_EM3), and CD27⁻CD28⁺ (T_EM4) within the 19z1⁺CD8⁺ subset from 4 donors. *P < .05, **P < .01, #P < .005 versus 19z1-ΔLNGFR.