Ectopic CEBPA induces differentiation of primary AML cells overexpressing MN1. BM samples of 2 inv(16) AML patients who overexpress MN1 (Figure 1F top panel, patients 5 and 6) were expanded for one day. Cells were transduced 3 times with MIG or MIG-CEBPA retroviruses during 2 consecutive days (retronectin-coated 24-well plates, 2 × 10⁵ cells/well; same conditions described in CD34⁺ cells with addition of 0.1 mg/mL DNAse I to prevent cell clumping). Half of the media was replaced every other day. Expression of the CD11b and CD14 was determined 2 or 4 days after the last transduction using FACS.