Apc-heterozygous mice developed ineffective erythropoiesis with erythroid dysplasia. (A) PB smears (left panels) and representative Giemsa-stained BM smears (right panels). (B) Flow cytometric analysis of BM and SP cells from representative Mx1-Cre⁺Apc^fl/+ and Mx1-Cre⁻Apc^fl/+ mice after pI-pC induction. The single-cell suspensions were simultaneously stained with anti-CD71 and anti-Ter119 antibodies. The numbers indicate the percentages of cells in each population. (C). Hematoxylin and eosin (H&E)–stained sections of adult SP (top panels) and Giemsa-stained spleen touch-preparations (bottom panels) from either control or anemic Apc heterozygous mice. The red arrows identify white pulp. Samples were obtained from both Mx1-Cre⁺Apc^fl/+ and Mx1-Cre⁻Apc^fl/+ mice 4 to 8 months after induction of the Apc deletion by pI-pC. Images were obtained using an Olympus microscope BX45 (Model U-DO3) equipped with an Olympus DP12 digital camera (A panels: 100× oil objective/1.25 NA, C top panels: 10× Plan air objective/0.3 NA, C bottom panels: 50× oil objective/0.9 NA), and processed using Microsoft PowerPoint.