LT-HSCs and ST-HSCs are expanded in the absence of a single allele of Apc. (A) Flow cytometric analysis of LSKs (Lin⁻ Sca-1⁺ Kit⁺) in primary mice. Comparison of the frequency of LSKs in Mx1-Cre⁺Apc^fl/+ mice and the control Mx1-Cre⁻Apc^fl/+ mice 2 weeks after induction (left panels) and 3 to 4 months after induction (right panels). The percentage of LSK cells is indicated (average ± SD of 3-5 animals).*P < .05; **P < .01. (B) Flow cytometric analysis of the proportion of long-term hematopoietic stem cells (LT-HSC)s, short-term hematopoietic stem cells (ST-HSCs), and multipotential progenitors (MPPs) in the LSK population from representative mice. (C) Total number of LT-HSCs, ST-HSCs and MPPs in BM from Mx1-Cre⁺Apc^fl/+ mice and the control Mx1-Cre⁻Apc^fl/+ mice 3 to 5 months after induction (average ± SD of 3 animals). *P < .05.