Analysis of in vitro colony-forming capacity, apoptosis, and proliferation of HPCs from Apc-heterozygous mice. (A) In vitro colony-forming assays. The number of BM CFU-GEMM, CFU-GM, CFU-G, CFU-M, and BFU-E was examined in Mx1-Cre⁺Apc^fl/+ or Mx1-Cre⁻Apc^fl/+mice 2 months after induction (mean ± SD of 3 animals). *P < .05. (B-C) Frequency of (B) apoptosis and (C) proliferation in gated HPCs (Lin⁻ Kit⁺ Sca-1⁻ Il-7R⁻) stained with Annexin V and DAPI, or DAPI, respectively, from Mx1-Cre⁺Apc^fl/+ and Mx1-Cre⁻Apc^fl/+ mice 3 to 4 months after pI-pC induction (mean ± SD of 3 animals). *P < .05; **P = .057.