LDL does not promote cell proliferation but affects hematopoietic differentiation in vitro. (A) B lymphoid-cell commitment is not altered by LDL (100 μg/mL) or HDL (100 μg/mL) exposure, defined as the ratio of CD19⁺ (B lymphocytes) to CD11b⁺ (myeloid) cells and assessed by flow cytometry. (B) LDL (100 μg/mL) or HDL (100 μg/mL) exposure does not change the total number of colonies formed in methylcellulose, a measure of CFUs. (C) LDL (100 μg/mL) and HDL (100 μg/mL) exposure increase the number of erythroid and macrophage CFU (CFU-E and CFU-M, respectively) and significantly decreases the number of granulocytic CFU (CFU-G). Inhibiting the cholesterol receptor SR-BI reverses the effect of LDL (*P < .05). These data were obtained from 2 separate experiments with consistent results.