![Figure 6. TNFR1 expression is not needed for T-cell priming in vivo. (A) Proliferation of 2.5 × 105 (gray and black bars) or 1.25 × 105 (□ and ) CD4+ T cells from either naive TNFR1+/+ or TNFR1−/− mice, or primed TNFR1+/+ or TNFR1−/− mice were stimulated for 72 hours with 5 × 105 of either unmodified ( and □) or TNCB-modified (■ and ) APCs; [3H] thymidine was added for the final 6 hours. (B) Frequency of IFN-γ–producing TNCB-specific CD4+ T cells, 5 × 105 (■), or 2.5 × 105 () T cells from either naive or sensitized TNFR1+/+ or TNFR1−/− mice were stimulated on anti–IFN-γ mAb-coated 96-well plates with 5 × 105 either unmodified APCs (, third column) or TNBS-modified APCs (■ and ). After 48 hours of incubation, ELISPOT assay was developed. Control T cells were stimulated with 10 μg/mL concanavalin A and APCs (□); 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/8/10.1182_blood-2008-11-187682/4/zh89990940510006.jpeg?Expires=1736058334&Signature=W-mtqJBAnKZSQPLYdpf1qQRfCViuMngBD1FrCe1mttqeXw-GODuYDjvKiKNiMlhHuK32FbvjH5H2bgWzIESrYpbuEpAhS6tFbwPUTwtsseArYSbezQRzlaLPyFHfFH-nbRu4B0~LMH1wITAoPu07rpHylZ5I7DXjVPeYNM2qy0s~YaN6GQ1r-RU6KOJlM-y-ZqnW9P8DOGcyou~M5muzmpOu5U0VhuYR38YbXNrRZGkUdI7b9RnOPTIQIMvXylY6mC0h3UJl6vv2iwkczB~UbL5fDLvY7JKqchWOHoGF8FxOPY4ER1dG-lYv2PG6q7TD79hi3lq8FFDt5-VOmqWghQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TNFR1 expression is not needed for T-cell priming in vivo. (A) Proliferation of 2.5 × 105 (gray and black bars) or 1.25 × 105 (□ and 
) CD4+ T cells from either naive TNFR1+/+ or TNFR1−/− mice, or primed TNFR1+/+ or TNFR1−/− mice were stimulated for 72 hours with 5 × 105 of either unmodified (
and □) or TNCB-modified (■ and ) APCs; [3H] thymidine was added for the final 6 hours. (B) Frequency of IFN-γ–producing TNCB-specific CD4+ T cells, 5 × 105 (■), or 2.5 × 105 () T cells from either naive or sensitized TNFR1+/+ or TNFR1−/− mice were stimulated on anti–IFN-γ mAb-coated 96-well plates with 5 × 105 either unmodified APCs (, third column) or TNBS-modified APCs (■ and ). After 48 hours of incubation, ELISPOT assay was developed. Control T cells were stimulated with 10 μg/mL concanavalin A and APCs (□); 3 independent experiments.
