Confirmation of the different types of acute leukemia by flow cytometric analyses. (A) Primary leukemic cells of all 3 histotypes were analyzed by flow cytometric analyses. Different antibody combinations and their conjugated fluorochromes are indicated. Leukemias are classified as pro-B ALL, B/T BAL, and mixed lineage leukemia (MLL). (B) CD45.2⁺ cells were used for the analysis and classification of secondary leukemias. The different antibody combinations are indicated. These analyses demonstrate engraftment and recapitulation of the primary disease phenotypes.