Characterization of miR-221/222–overexpressing MEC1 cell lines. MEC1 cells were infected with miR-expressing LV vectors. Infected cells were sorted, and overexpression of miR-221/222 was assessed by real-time PCR (A). Control and miR-transduced cells were plated at the concentration of 5 × 105/mL and counted by Trypan-blue exclusion at the indicated time points (B left). The experiment was repeated after synchronization by double thymidine block and plating (B right). Synchronized cells were stained with propidium iodide at the indicated time points after block release, and cell-cycle distribution was analyzed by flow cytometry. Percentage of cells in G1, S, and G2/M phase of the cell cycle is shown (C). Graph represents the average of 3 different experiments (mean + SD). Differences were analyzed by 2-way analysis of variance and Bonferroni test.
