Expression of p27 and miR-221/222 inversely correlate in CLL cells from patients. Western blot experiment with the use of leukemic cells of 5 representative CLL cases shows variable, although readily detectable, levels of expression of p27 protein (A top). β-Actin is used as loading control. Quantification by densitometric analysis expression is reported as p27/Actin ratio between OD (A bottom). Quantitative PCR analysis for miR-221 and miR-222 (TaqMan miRNA assay) on the same patients are reported (B (top and bottom, respectively). (C) The negative correlation trendline between miR-221 (left) or miR-222 (right) and p27 in 38 patients with CLL.