miR-29 family members target to CDK6. (A) Sequence alignment of the miR-29a/b/c seed sequences with 2 regions of the CDK6 3′-UTR. Mutants of pmiR-CDK6 3′-UTR 1m/2m are labeled in red. (B) Inverse correlation between expression of CDK6 (left panel) and miR-29a/b/c (right panel) in indicated cell lines. Ectopic expression of miR-29 (C) in Mino cells reduces CDK6 protein (D) and mRNA (E) levels. Each experiment was repeated 3 times. Error bars represent SD.