miR-29 interacts with the CDK6 3′-UTR. (A) Luciferase activity of pmiR-CDK6-1-3′-UTR and pmiR-CDK6-2-3′-UTR, but not their mutants, is low in miR-29-high Jeko-1 and high in miR-29-low Mino cells. Ectopic expression of miR-29c in both HEK293T (B) and Mino (C) cells inhibits luciferase activity of pmiR-CDK6-1-3′-UTR and pmiR-CDK6-2-3′-UTR but not their mutants. Knockdown of miR-29 increases the luciferase activity in Jeko-1 cells (D). Each experiment was repeated 3 times. Error bars represent SD.