Characteristics of B-lymphoma cells generated from the transgenic mice. (A) PCR analysis of immunoglobulin gene rearrangement in splenic B cells of wild-type mice and B-lymphoma cells of transgenic mice. Lane 1 shows J558-J_H4; lane 2, 7183-J_H4; lane 3, Q52-J_H4; lane 4, D-J_H4; and lane 5, distilled water. (B) Morphologic appearance of B-lymphoma cells in vitro (left panel shows bright field in culture; right panel, May-Gruenwald-Giemsa staining of a cytospun sample; 63×/1.4 NA oil objective). Bars represent 10 μm. (C-D) Electrophoresis of genomic DNA (C) and propidium iodide staining (D) of untransduced or Bcl-x_L–transduced B-lymphoma cells. (E) Flow cytometric analysis of marrow, spleen, and lymph node cells in NOD/SCID mice that underwent transplantation with B-lymphoma cells at 4 weeks after transplantation. (F) Karyotype analysis of the B-lymphoma cells by Giemsa staining (100×/1.4 NA oil objective). (G) The number of chromosomes of B-lymphoma cells. MW indicates molecular weight marker in panels A and C.