Hematologic abnormality in mice that received transplants of KL cells genetically modified to express D3 and Bcl-xL. (A) RT-PCR analysis of D3 and Bcl-xL expression in peripheral blood cells from mice that underwent transplantation with D3-transduced or D3- and Bcl-xL–transduced KL cells. Left and right panels show D3 and Bcl-xL expression, respectively. β-actin is used as an internal control. D3 mice indicates mice that received transplants of D3-transduced KL cells; D3/Bcl-xL mice, mice that received transplants of D3- and Bcl-xL–transduced KL cells; and MW, molecular weight marker. A vertical line has been inserted to indicate repositioned gel lanes of β-actin and Bcl-XL. (B-C) A total of 5 mice received transplants of KL cells transduced with both D3 and Bcl-xL. The percentages of leukocytes expressing Mac1 (B) and the total number of leukocytes (C) in the peripheral blood are shown. Each open or filled symbol represents a mouse with or without hematologic abnormality, respectively.
