Analysis of mice affected with B lymphoma after transplantation with D3- and Bcl-xL–transduced KL cells. (A-B) Morphologic appearance of spleen, lymph nodes (A), and peripheral blood cells (May-Gruenwald-Giemsa staining; B) in mice that received transplants of D3- and Bcl-xL–transduced KL cells at 40 weeks after transplantation (63×/1.4 NA oil objective). Bar in panel B represents 10 mm. (C) Flow cytometric analysis of marrow or peripheral blood cells (left and middle panels, respectively). D3 expression in Mac1− or Mac1+ cells in B220+ population was further analyzed (right panel). (D) Survival analysis of mice that underwent transplantation with huKO-transduced (○), D3-transduced (●), Bcl-xL–transduced (□), or D3- and Bcl-xL–transduced KL cells (■). BMT indicates BM transplantation. *P < .01 compared with the other groups.
