Different parameters affect the ability of polyP to modulate lysis. Fibrin clots were formed from fibrinogen (2.4μM), plasminogen (0.24μM), and tPA in the absence (○) and presence (●) of polyP₆₅ (325μM) by adding thrombin and CaCl₂ (A). The concentration of thrombin used to induce clotting was varied from 0.016 U/mL to 5 U/mL with constant CaCl₂ (5mM) and tPA (20pM) concentrations. (B) The concentration of CaCl₂ was varied from 0 to 20mM in the presence of constant thrombin (0.25 U/mL) and tPA (20pM) concentrations. (C) The tPA concentration was varied from 10 to 500pM in the presence of constant thrombin (0.25U/mL) and CaCl₂ (5mM) concentrations. All the results are expressed as the mean ± SEM time to 50% lysis (n = 4).