PolyP only affects lysis if present during polymerization of fibrinogen. (A) Fibrin clots were formed from fibrinogen (2.4μM), plasminogen (0.24μM), and tPA (20pM) in the absence (gray line) and presence (black line) of polyP₆₅ (325μM) by adding atroxin (10 μg/mL) and CaCl₂ (5mM; n = 4). (B) Soluble fibrin was repolymerized in the presence of plasminogen (0.24μM) and tPA (20pM) in the absence (gray line) and presence (black line) of polyP ₆₅ (325μM; n = 5). (C) Clots were generated with purified fibrinogen clotted with (black line) and without (pale gray line) polyP₆₅ (325μM). Clots were incubated for 30 minutes at 37°C before overlaying with a mixture of plasminogen (0.55μM) and tPA (10nM) with (dark gray line) and without polyP₆₅ (325μM; n = 8). Results are normalized and expressed as the mean ± SEM.