![Figure 1. A truncated active SphK2 (t-SphK2) is released into the supernatant of apoptotic Jurkat cells. (A-B) Jurkat cells remained as controls or were treated with Sts as described in “Induction and detection of apoptosis.” (A) Western analysis of SphK2 in subcellular fractions (nuclear [Nuc], membrane [Mem], cytosolic [Cyt]) or the cell supernatant (Sup). The histogram shows quantification of Western data as means ± SEM from 3 independent experiments. (B) SphK2 activity in Jurkat cell lysates (■) and cell supernatants (□). Data are means ± SEM from 4 independent experiments. *P < .05 compared with unstimulated controls. (C) Western analysis of SphK2 expression in whole-cell lysate (L) and supernatant (S) from Jurkat and SphK2-deficient Jurkat cells that were untreated or treated with Sts. (D) Blots show expression of SphK2 in cytosol and supernatants of control or UV-irradiated (10 mJ/cm2) Jurkat cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/115/17/10.1182_blood-2009-10-243444/4/zh89991051990001.jpeg?Expires=1735563349&Signature=DOWvUkHDuo~LZ2bERyhHSvim4~698RFnT36V6hPQdy1LptrBIDvjPy5ZeA9Y0YiH3FgaqRkc95On53XI~BFb~Sw1tuo0dWOttGy1a1a~5ypTXOWY88e-xZw1lUp33I1OL6-cYDqTL9iQFuVWDu2y9F6-hXPs1jQGnYMXCTj~CT~zx0tsc-imomTEKU6Y0cVdBP2sm4wxg~-LAaZIt70yn70AWaQOgLHC~BJWMScoXVcAf3poubC8ArIbTn16KYR67VfJ3rYOpvTpCqZsgPUkb2vfnqhbMbfl7vetMAop4Xq33ByiDpsfHfBYPCIDViEjUeUeu2Xyx6wvod9Kff9EOg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
A truncated active SphK2 (t-SphK2) is released into the supernatant of apoptotic Jurkat cells. (A-B) Jurkat cells remained as controls or were treated with Sts as described in “Induction and detection of apoptosis.” (A) Western analysis of SphK2 in subcellular fractions (nuclear [Nuc], membrane [Mem], cytosolic [Cyt]) or the cell supernatant (Sup). The histogram shows quantification of Western data as means ± SEM from 3 independent experiments. (B) SphK2 activity in Jurkat cell lysates (■) and cell supernatants (□). Data are means ± SEM from 4 independent experiments. *P < .05 compared with unstimulated controls. (C) Western analysis of SphK2 expression in whole-cell lysate (L) and supernatant (S) from Jurkat and SphK2-deficient Jurkat cells that were untreated or treated with Sts. (D) Blots show expression of SphK2 in cytosol and supernatants of control or UV-irradiated (10 mJ/cm2) Jurkat cells.
