Prophylactic vaccination with polyUs21 prevents tumor growth. C57BL/6 mice were challenged intravenously with B14.3 melanoma cells 30 days after vaccination with OVA in combination with the indicated TLR agonists. Control mice were injected with HEPES-buffered saline or with a mixture of OVA and DOTAP. The tumor burden was determined 18 days after tumor challenge. Each dot represents an individual house. Pooled data from at least 3 independent experiments are shown.