Adaptive immune responses induced by immunization with polyUs21 are TLR7 dependent. (A) C57BL/6 (●) and TLR7 KO (○) mice were immunized with a combination of OVA and the indicated TLR agonists. PolyUs21 was administered in form of complexes with DOTAP, and control mice received a combination of OVA and DOTAP. In vivo CTL assays were performed 7 days after immunization. Data are a pooled from 4 independent experiments. (B) OVA-specific serum titers of IgG1 and IgG2c antibodies were determined by ELISA in wild-type and TLR7 KO mice immunized with (solid lines) or without (dotted lines) TLR agonists. Serum dilution curves represent the compiled data of 3 independent experiments with the SEM indicated.