CTL responses to polyUs21 are dependent on signaling through IFNRI. (A) 129 Sv mice (●) and IFNRI KO (○) were vaccinated with egg white preparation as a source of OVA in combination with the indicated TLR agonists. In vivo CTL killing assays were performed at day 7 after vaccination. Data are pooled from 2 independent experiments. (B) C57BL/6 mice were treated with egg white preparation in combination with IFN-α (104 or 105 U/mouse) in either the absence (●) or presence (○) of 100 μg of R848. On day 7 after vaccination, the in vivo CTL killing assay was performed. Data show the mean ± SEM from 3 independent experiments. (C) C57BL/6 mice were treated with PDC-depleting (○; clone 927) or isotype control antibody (●). Upon depletion of PDC, mice were vaccinated with OVA in combination with the indicated TLR agonists. The in vivo CTL killing assay was performed at day 7 after vaccination. The graph contains pooled data from 3 independent experiments with the SEM indicated.
