Differential CD85j expression on human PBMC subsets. (A) Representative histograms showing CD85j expression on NK cells, CD4 T cells, CD8 T cells, B cells, and monocytes. (B) Frequencies of CD85j⁺ cells within subpopulations are shown as mean + SD of 12 donors. (C) CD85j expression on CD8 T-cell subsets. Histograms (bottom) show CD85j expression on the CD8 T-cell subpopulations based on the expression of CCR7 and CD45RA as shown in the dot plot at top. Numbers in the histograms (bottom) indicate the percentage of cells expressing CD85j. (D) CD85j expression on peripheral transitional B cells. Histograms (right) show CD85j expression on the populations defined in density plot to the left (gated on CD19⁺CD20⁺ cells). T1 indicates early transitional B cells; and T2, late transitional B cells. (E) CD85j expression on peripheral naive and memory B cells. Histograms (right) show CD85j expression on the populations defined by the quadrants in dot plot to the left (gated on CD19⁺ cells). (F) CD85j cell surface densities (MFI indicates mean fluorescence intensity) by flow cytometry on the CD85j⁺ cells are shown as mean MFI + SD of 12 donors. (G) CD85j expression on monocytes and B cells are compared by Western blot. Blot is representative of comparisons of 4 donors.