LILRB1 mRNA levels does not account for different subset-specific CD85j protein expression. LILRB1 transcripts were quantified by qRT-PCR in RNA from magnetic bead–separated CD8 T cells, CD19 B cells, and CD14 monocytes, and NK cells were purified by negative selection. (A) Results for a primer set within exon 8 are shown as mean transcript numbers + SDs of 10 to 12 donors per group relative to 2 × 105 β-actin copies. Compared with protein expression, transcript numbers in monocytes were disproportionately low. (B) LILRB1 transcripts were compared for exon 1 to 3 and exon 8 sequences. Results are shown as a scatter plot for CD8 T cells, B cells, and monocytes. (C) Transcript comparisons are quantified as the ratio of LILRB1 exon 1 to 3 to exon 8 copies. Results are shown as mean + SD of 10 to 12 donors per group. NK-cell data are from 3 donors.
