Expansion and differentiation of erythroid cells from different developmental stages. (A) Schematic representation of the 2-phase erythroid culture model showing the experimental time frame and additions to the culture medium during expansion and differentiation. (B) Total cell numbers derived from 105 CD34+ cells from cytokine mobilized PB, adult BM, CB, or FL over the initial 12 days of culture. (C) Flow cytometric analysis for expression of CD71 (transferrin receptor) and CD235 (glycophorin A) where the percentages indicate the proportion of cells considered positive and (D) morphology of Wright-Giemsa–stained cytocentrifuge preparations (original magnification × 60) after 20 days of culture. (E) Cellulose acetate Hb electrophoresis of erythroblast lysates from cultured PB, BM, CB, and FL CD34+ cells and whole blood (WB) from an adult after 12 or (F) 16 days of culture.
