Effect of ϵ-ACA and carboxipeptidase B on Lys- and Glu-plasminogen activation by cellular uPA. THP-1 cells (10⁵/well) were incubated with 500nM Lys- () or Glu-plasminogen (□) in medium alone or supplemented with ϵ-ACA (5 or 25mM) and 0.75mM CBS0065. Carboxypeptidase B, CpB (50 μg/mL), pretreated THP-1 cells were incubated with plasminogen and CBS0065. Rate of plasmin formation (A) and amount of cell-associated plasmin (B) were detected as indicated in Figure 2. Bars represent the amount of plasmin formed or associated to the cells (mOD/minute) versus the concentration of ϵ-ACA or after CpB treatment (mean ± SEM; n = 3). Significant changes compared with Lys-Pg (*P < .05)/Glu-Pg (§P < .05) alone.