CD8⁺ and CD20⁺ lymphocyte depletion in SIV-infected AGMs did marginally affect the number of memory CD4⁺ T cells and Ki-67⁺ memory CD4⁺ T cells in peripheral blood. CD4⁺ T cells of control Ab-treated AGMs (A-D) and CD8⁺ and CD20⁺ lymphocyte-depleted AGMs (B-E) were stained with anti-CD28 and anti-CD95 to determine naive (CD95⁻) and memory (CD95⁺) CD4⁺ T-cell subsets. The fold change in memory CD4⁺ T-cell numbers (A-C) and the fold change in Ki-67⁺ memory CD4⁺ T-cell numbers (D-E) were calculated for each time point compared with the median of 3 preantibody injection time points. (C) The median fold change in memory CD4⁺ T cells from the 6 control Ab-treated AGMs (solid line, filled symbols) and the 6 CD8⁺ and CD20⁺ lymphocyte-depleted AGMs (solid line, open symbols) are shown. (F) The median fold change in Ki-67⁺ memory CD4⁺ T cells from the 6 control Ab-treated AGMs (solid line, filled symbols) and the 6 CD8⁺ and CD20⁺ lymphocyte-depleted AGMs (solid line, open symbols) are shown. *Single time point with a significant difference (Mann-Whitney test) between the control group and the antibody-treated group of animals. No significant differences were detected between the control and antibody-treated groups at any of the other time points. p.i. indicates postinfection.