Global screening of the F11 splicing pattern. The existence of physiologic F11 alternatively spliced transcripts was first assayed in HepG2 cells. (A) Schematic representation of the FXI cDNA. The position of the primer couples used for each RT-PCR assay (indicated with i-iv), and the expected length of the corresponding amplification products are also indicated. (B) RT-PCR products obtained with each primer couple (i-iv) separated on a 2% agarose gel. Lane M indicates molecular weight marker (pUC8-HaeIII). *AS products whose existence was confirmed by at least one sequenced recombinant clone. No clone with an insert compatible with the length of the faint upper band in lane ii was found.