Characterization of F11 alternatively spliced transcripts. AS of the F11 pre-mRNA was analyzed in HepG2 cells (A), human liver (B), and platelets (C) by RT-PCR using primer couples specific for each identified splicing isoforms (Δ3-Δ13). Lane M indicates molecular weight marker (pUC8-HaeIII). (D) Schematic representation of the F11 gene and of its splicing pattern. Boxes represent exons; lines, introns; broken lines, splicing events; hatched lines, ASs; dead-end sing, out-of-frame splicings leading to the introduction of a PTC (star); and stop sign, physiologic stop codons.