Binding of RUNX1 to ALOX12 promoter region in vivo. ChIP using control IgG (column 1) and RUNX1 antibody (column 2). Column 3 shows PCR of total input DNA. Data are PCR amplification of ALOX12 promoter regions encompassing sites 1A and 1B (−1679/−1376 nt), site 2 (−840/−600 nt), site 3 (−597/−360 nt), and glyceraldehyde-3-phosphate dehydrogenase.