Effect of siRNA RUNX1 knock down on ALOX12 promoter activity and protein. (A) Effect on ALOX12 promoter activity. HEL cells were cotransfected with RUNX1 or mock siRNA and ALOX12 luciferase-reporter construct (−1593/−1). Bar graphs represent activity as mean ± SD of 3 experiments. (B) Western blotting analysis of RUNX1, 12-LO, and actin in HEL cells transfected with mock or RUNX1 siRNA. Data are representative of 3 experiments.