Effect of transient overexpression of RUNX1 on ALOX12 promoter activity. (A) Effect on ALOX12 promoter activity. HEL cells were cotransfected with RUNX1-pCMV6 expression vector (■), empty pCMV6 vector (), or neither (□), along with ALOX12 luciferase-reporter construct (−1593/−1), wild-type, or with mutations in sites 1A, 1B, 2, or 3. Reporter activity was measured at 48 hours. Bar graphs represent activity as mean ± SD of 3 experiments. (B) Western blotting analysis of RUNX1 and actin (control) in HEL cells transfected with pCMV6 vector alone or with RUNX1-pCMV6 vector.