Thymocyte development in GIMAP1/CD2-cKO mice. (A) Representative FACS plots of thymocytes from GIMAP1fl/flhCD2-iCre− and GIMAP1fl/flhCD2-iCre+ mice stained for CD4, CD8, and TCR (the latter to exclude CD8+ intermediate SP cells). (B) Mean numbers (± SD) of thymocyte subsets (■ indicates GIMAP1fl/flhCD2-iCre−, n = 22; □, GIMAP1fl/flhCD2-iCre+, n = 18). (C) Mean numbers (± SD) of mature (Qa2hiCD24−) CD4 and CD8 SP thymocytes (■ indicates GIMAP1fl/flhCD2-iCre−, n = 6; □, GIMAP1fl/flhCD2-iCre+, n = 4).