Peripheral B-cell analysis. Splenocytes from GIMAP1fl/flhCD2-iCre− and GIMAP1fl/flhCD2-iCre+ mice were stained for flow cytometric analysis to identify B-cell subsets. (A) Representative FACS plots for each genotype. The numbers given inside the component panels in A are the percentages of lymphocyte events contained within the indicated gates. (B) Mean numbers (± SD) of B-cell subsets (■ indicates GIMAP1fl/flhCD2-iCre−, n = 6; □, GIMAP1fl/flhCD2-iCre+, n = 6). *P < .05; **P < .01; ***P < .001 (unpaired 2-tailed Student t test). FSC indicates forward scatter; and FO, follicular.