Analysis of GIMAP protein expression in B- and T-cell development. BM cells (A), splenic B cells (B), and thymocytes (C) from C57BL/6 mice were FACS-purified into subsets as described in “Fluorescence-activated cell sorting.” Expression of GIMAP4, GIMAP5, GIMAP1, GIMAP6, and GIMAP9 was examined by Western blot, with β-actin as control. *Parentheses surround nonspecific bands caused by the secondary anti–rat IgG reagent.