CD4 T-cell depletion reduces the self/TERT-specific CD8 T cells in lv-hTERT–immunized mice. HHD mice (4/group) were treated either with anti-CD4 monoclonal antibody (GK1.5; CD4 depleted) or with saline (control), 3 days before immunization. Ten (A) or 30 days (B) later, spleen-isolated CD8 T cells were cultured with p572 peptide or medium. The p572-specific CD8 T cells were detected ex vivo by IFN-γ ELISpot.