Knockdown of PRMT1 inhibits the recruitment of the transcription preinitiation complex to the β-globin locus. (A) ChIP assays of TBP at the HS2 enhancer and β^maj-globin promoter in MEL cell lines transduced with the vector control or shPRMT1 in the presence of 1.5% DMSO for 3 days. The precipitated DNA was analyzed by real-time qPCR. The MyoD promoter and the GAPDH promoter were also analyzed as inactive and active promoter controls, respectively. (B) ChIP assays of TAF1 at the HS2 enhancer and β^maj-globin promoter in MEL cell lines transduced with the vector control or shPRMT1 in the presence of 1.5% DMSO for 3 days. (C) ChIP assays of RNA Pol II at the HS2 enhancer and β^maj-promoter comparing the vector control and shPRMT1-transduced MEL cell lines in the presence of 1.5% DMSO for 3 days. Significant difference by Student t test: *P < .05, **P < .01. Shown are the mean ± SDM of 3 independent experiments.