Melt-curve, sequence, and Ipsogen MutaScreen kit results. Results of heterozygous control (top) and patients no. 1 (middle) and no. 2 (bottom). Melting temperatures (Tm) of wild-type and JAK2 mutations are indicated. Sequence analysis of 617V>F heterozygote compared with patients no. 1 (homozygous 617V>F/618C>R; 1849G>T, 1851C>T, 1852T>C) and no. 2 (heterozygous 617V>F/618C>F; 1849G>T, 1853G>T). Using the Ipsogen kit, no signal is obtained in patient no. 1, who is homozygous mutant, due to failure of the mutant probe to bind to the mutant allele. Patient no. 2, who is heterozygous, genotypes as wild-type because the mutant probe does not bind to the mutant allele.