Iron status of Tfr2-deficient mice. (A) Transferrin saturation values in the 3 mouse models at 10 weeks of age. Transferrin saturation was measured on 5 animals for each strain. ***P < .001 versus WT. Bars represent SD in the analyzed animals group. (B) LIC in Tfr2-targeted mice at different ages. Statistical differences between LIC values of the different Tf2-targeted mice versus WT sib pairs at the same ages: *P < .05, **P < .01, and ***P < .001. Statistical difference between LIC values of Tfr2 KO and LCKO-KI mice at 22 weeks of age is also reported (P < .001). Bars represent SD in the analyzed animals group.