Figure 1
Anti–third-party CTLs are excluded from the LNs, and do not colocalize with HTCs in the early time points after BM transplantation. BALB/c mice were conditioned with 8 Gy of lethal TBI and infused with different cell mixtures as follows: (A) C57BL/6-NUDE BM (4 × 106). (B) C57BL/6-NUDE BM + HTCs (1 × 104) + DIR-labeled syngeneic naive HTCs (7 × 106). (C) C57BL/6-NUDE BM + HTCs (1 × 104) + DIR-labeled naive allogeneic CB6-derived CD8+ T cells (7 × 106). (D) C57BL/6-NUDE BM + HTCs (1 × 104) + DIR-labeled allogeneic CB6-derived anti–third-party CTLs (7 × 106). Images were taken 36 hours after transplantation using IVIS. A representative mouse (of 6 mice in 2 independent experiments) is shown for each group.
