Fully allogeneic anti–third-party Tcms are depleted of GVH reactivity and support engraftment of TDBM allografts. (A-B) Supralethally irradiated (11 Gy) C3H mice were radioprotected with 5 × 106 BALB/c-NUDE BM cells in the presence or absence of 5 × 106 or 2 × 106 BALB/c-derived CD8+ purified Tcms (BM + Tcm 5 or BM + Tcm 2, respectively) or naive cells (BM + naive 5 or BM + naive 2, respectively). The GVH reactivity of the Tcms or the naive cells was reflected by survival percentage (A) or by average weight change (B) during 100 days after transplantation. Data represent averages of 3 independent experiments, with at least 5 mice for each group, in each experiment. **P < .01 compared with the group of mice that received only BM. (C) Lethally irradiated (10 Gy) C3H mice received 1.25 × 104 syngeneic HTCs. Mice then received a transplant of 3 × 106 BALB/c-NUDE BM cells (BA-NU BM) in the presence or absence of 5 × 106 BALB/c CD8+ purified Tcms or 1 × 107 BALB/c CTLs. Data were pooled from 3 independent experiments.
