ALDH⁺ DCs from the MLNs and the LP display a heterogeneous surface phenotype. Light-density cells isolated from MLNs (A) and from the small intestine LP (B) were incubated with ALDEFLUOR and analyzed by flow cytometry for expression of CD11c, MHCII, CD11b, CD103, and CD45 and for ALDH activity. Dot plots correspond to nonautofluorescent cells with a FSC^highSSC^high profile characteristic of DCs and from which neutrophils were gated out using Ly6G and Gr-1 staining. Positioning of the ALDH⁺ gate is based on incubation in the presence of DEAB (supplemental Figure 3). For MLNs, the CD11c^{inter to high}MHCII^high and the CD11c^highMHCII^inter gates correspond to mDCs and lymphoid tissue–resident DCs, respectively. In the case of LP, the gates corresponding to macrophages (MFs) and to DCs are specified in supplemental Figure 2A. Numbers in outlined areas indicate percentage of cells. Data are representative of at least 3 separate experiments involving groups of 3 to 6 mice.