Reduced levels of ALDH activity in DCs from liver, thymus, and spleen. Light-density cells isolated from liver (A), thymus (B), and spleen (C) were incubated with ALDEFLUOR and analyzed by flow cytometry for the expression of CD11c, MHCII, CD11b, CD103, CD45, SIRPa, and CD8a and for ALDH activity. Dot plots correspond to nonautofluorescent cells from which neutrophils were gated out using Ly6G and Gr-1 staining. Positioning of the ALDH⁺ gate is based on incubation in the presence of DEAB (supplemental Figure 3). The gates corresponding to liver Kupffer cells, liver DCs, thymic MHCII^high DCs, splenic cDCs, splenic pDCs, and splenic MFs are specified in supplemental Figure 2E through G. Numbers in outlined areas indicate percentage of cells. Data are representative of at least 3 separate experiments involving groups of 3 to 6 mice.