Figure 4
Figure 4. ATRA does not affect PML-RARα degradation or the dephosphorylation of FOXO3A in ATRA-resistant NB4/RA cells. NB4/RA cells were incubated with 1.0μM ATRA or a final volume of 0.1% ethanol for 48 hours. (A) Cells were fixed on poly-l-lysine–treated glass slides. After permeabilization, cells were incubated with anti-PML antibody and with Alexa488-conjugated goat anti–mouse IgG. Imaging analysis was performed with an Olympus IX70 microscope. Scale bar represents 10μm. (B) Total protein was extracted and submitted to Western blotting with antibodies for each indicated antigen. (C) TRAIL mRNA was detected by quantitative RT-PCR using TaqMan probes for TRAIL and GAPDH. Quantification was performed using the relative standard curve method. Results are presented as the mean ± SE of 3 independent experiments. **P < .01 compared with ethanol control.

ATRA does not affect PML-RARα degradation or the dephosphorylation of FOXO3A in ATRA-resistant NB4/RA cells. NB4/RA cells were incubated with 1.0μM ATRA or a final volume of 0.1% ethanol for 48 hours. (A) Cells were fixed on poly-l-lysine–treated glass slides. After permeabilization, cells were incubated with anti-PML antibody and with Alexa488-conjugated goat anti–mouse IgG. Imaging analysis was performed with an Olympus IX70 microscope. Scale bar represents 10μm. (B) Total protein was extracted and submitted to Western blotting with antibodies for each indicated antigen. (C) TRAIL mRNA was detected by quantitative RT-PCR using TaqMan probes for TRAIL and GAPDH. Quantification was performed using the relative standard curve method. Results are presented as the mean ± SE of 3 independent experiments. **P < .01 compared with ethanol control.

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