Naive CD8⁺ T cells specific for HIV Gag p17_77-85 can be detected in healthy donors. (A) TAME was performed using HIV Gag p17_77-85 and MART1_26-35 HLA-A2.1 tetramers labeled with PE. Percentage of tetramer-specific T cells are indicated before and after enrichment. (B) Enriched tetramer-positive cells were further analyzed using anti-CD45RA, anti-CCR7, anti-CD11a, and anti-CD27 antibodies. Cells were gated on CD45RA and assessed for their expression of other naive T-cell markers. Dot plots shown in black represent the phenotype of tetramer-positive populations; overlaid in gray, bulk CD8⁺ T cells are shown as a reference population. All percentages refer to tetramer-positive population.