MART1_26-35–specific cells specifically mature under stimulation by pulsed DCs. PBMCs were incubated with anti-CD8 microbeads, and enriched cells were cocultured for 7 days with DCs loaded with MART1_26-35 peptide. T cells were then harvested and simultaneously stained with MART1_26-35 and HIV Gag p17_77-85 tetramers, and cell phenotyping was performed on each tetramer-positive population. Data are representative of 2 independent experiments.