American Society of Hematology

Figure 5

$Figure 5. TAME permits detection of 1 cell in 107 CD8+ T cells. Titrated amounts of a CTL clone specific for HIV Gag p1777-85 were dispensed into known amounts of PBMCs of a healthy donor. Corresponding frequencies of spiked cells are indicated (input frequency). Samples were then stained with HIV Gag p1777-85 HLA-A2.1 tetramer labeled with PE, and the ability to detect the CTL clone was determined in the pre-enriched and enriched fractions. The limit of detection was determined to be ∼ 10−5 before and ∼ 10−7 after TAME. Measured frequency after enrichment is indicated for comparison. Results are representative of 2 independent experiments.$

TAME permits detection of 1 cell in 10⁷ CD8⁺ T cells. Titrated amounts of a CTL clone specific for HIV Gag p17_77-85 were dispensed into known amounts of PBMCs of a healthy donor. Corresponding frequencies of spiked cells are indicated (input frequency). Samples were then stained with HIV Gag p17_77-85 HLA-A2.1 tetramer labeled with PE, and the ability to detect the CTL clone was determined in the pre-enriched and enriched fractions. The limit of detection was determined to be ∼ 10⁻⁵ before and ∼ 10⁻⁷ after TAME. Measured frequency after enrichment is indicated for comparison. Results are representative of 2 independent experiments.

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