Enumeration of epitope-specific CD8⁺ T cells reveals conserved precursor frequency among healthy donors. After performing TAME with MART1_26-35, NY-ESO1_157-165, HIV Gag p17_77-85, HCV NS3_1406-1415, HCV Core_132-140, or CMVpp65_495-503 HLA-A2.1 tetramers, CD8⁺ T cells specific for each of these epitopes were enumerated. CMVpp65_495-503 in seropositive persons and influenza A–M1_58-66 tetramer staining were used as positive controls. Each point represents a single healthy person. CMV_pp65(sn) and CMV_pp65(sp) indicate seronegative and seropositive donors, respectively, as confirmed by serologic testing.