Analysis of immunologic organs of B6.GT mice. (A) Gross thymus, lymph node, and spleen organ size in age-matched B6.WT, B6.TRAIL^−/−, B6.gld/gld, and B6.GT mice. Figures show 1 mouse per strain, with 5 or more mice per strain analyzed, as indicated in lymph node weight data. (B) Percentage of conventional CD3⁺B220⁻ T cells (R1), as well as CD3⁺B220⁺ (R2) CD4⁻CD8⁻“double negative” T cells (DN T cells) and CD3⁻B220⁺ B cells as CD21⁻CD23⁺ follicular (Fo) B cells, CD21⁺CD23⁻ marginal zone (MZ) B cells, and CD21⁻CD23⁻ nonfollicular (NF) B cells in spleen and axillary lymph nodes of 3 age- and sex-matched B6.WT, B6.TRAIL (B6.T), B6.gld/gld (B6.G), and B6.gld/gld.TRAIL^−/− (B6.GT) mice. FACS data (dot plots) shown are from 1 representative mouse of 3 age- and sex-matched mice per strain. (C-D) Total leukocyte cellularity and absolute numbers of T cell, B cell, and DN T cell leukocyte subsets calculated from dot plots in panel B. Numbers of non T and B cells were determined by gating first on CD3⁻B220⁻ cells and subsequently on Gr1⁺, CD11c⁺, or NK1.1⁺ cells, whereas NKT cells were defined from within the CD3⁺B220⁻ cells that expressed NK1.1. Data shown are mean plus or minus SD of each tissue from 3 mice per strain. Statistical significance was assessed using Mann-Whitney analysis, with differences between B6.gld/gld or B6.GT compared with B6.WT (*P < .05) and between B6.G and B6.GT (**P < .05).