Survival and breeding potential. (A) Mice were monitored from birth, until their sudden death, or until they were killed (having become moribund), in full accordance with Western Sydney Area Health Service institutional animal ethics guidelines. Kaplan-Meier graphs were plotted with survival data from 10 B6.WT, 12 B6.TRAIL^−/−, 21 B6.gld/gld, and 25 B6.GT mice. Data were analyzed with log-rank test that indicated a statistically significant difference of P < .001 between B6.G and B6.GT. A statistically significance difference (P = .012) was also evident between female versus male B6.GT mice, but no significant difference was found for male versus female B6.gld/gld (P = .233). (B) Breeding potential was assessed by reviewing the number of litters per breeder pair and number of mice per litter, with data from long-term breeding records over 4 years. Male and female mice were mated at approximately 6 to 7 weeks of age, with pups weaned at approximately 3 weeks of age, and male mice were left with their female partners to allow postpartum mating.